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Objective:To evaluate the short-term effect of robotic pancreaticoduodenectomy (RPD) and laparoscopic pancreaticoduodenectomy (LPD) in the treatment of ampullary and periampullary tumor systematically.Methods:A systematic search of the PubMed, Embase and Cochrane library database using the key words "pancreaticoduodenectomy, duodenopanreatectpmy, whipple, laparoscopic, robotic, Da Vinci" . A systematic search of the Sinomed, Wangfang, VIP and CNKI databases including the key words "胰十二指肠切除" , "腹腔镜" , "机器人" , "达芬奇" . To investigate the differences of the conversion rate of laparotomy, incidence of postoperative complications, and the postoperative hospital stay between the RPD group and the LPD group. The software RevMan5.3 was used in this meta-analysis.Results:A total of 4 retrospective cohort studies and 1 001 patients were included in this meta-analysis, including 451 patients in the RPD group and 550 patients in the LPD group. The results of the meta-analysis showed that there were significant differences between RPD group and LPD group for the conversion rate laparotomy ( OR=0.35, 95% CI: 0.24-0.50, P<0.05). There were no significant differences between RPD group and LPD group for the incidence rate of overall complication( OR=1.23, 95% CI: 0.95-1.58, P>0.05), hemorrhage ( OR=0.71, 95% CI: 0.50-1.00, P>0.05), pancreatic fistula ( OR=1.09, 95% CI: 0.80-1.49, P>0.05), delayed gastric empty ( OR=0.81, 95%CI: 0.57-1.14, P>0.05) and hospital stay after surgery ( WMD=-2.87, 95% CI: -1.44-1.70, P>0.05). Conclusions:RPD is as safe as LPD, with the same complication rate. Therefor it is worthy for further application in medical institutions with relevant conditions.
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In current study, we aimed to investigate whether the gentiopicroside (GPS) derived from Gentiana manshurica Kitagawa could block the progression of alcoholic hepatic steatosis to fibrosis induced by chronic ethanol intake. C57BL/6 mice were fed an ethanol- containing Lieber-DeCarli diet for 4 weeks. LX-2 human hepatic stellate cells were treated with GPS 1 h prior to transforming growth factor-β (TGF-β) stimulation, and murine hepatocyte AML12 cells were pretreated by GPS 1 h prior to ethanol treatment. GPS inhibited the expression of type I collagen (collagen I), α-smooth muscle actin (α-SMA) and tissue inhibitor of metal protease 1 in ethanol-fed mouse livers with mild fibrosis. In addition, the imbalanced lipid metabolism induced by chronic ethanol-feeding was ameliorated by GPS pretreatment, characterized by the modulation of lipid accumulation. Consistently, GPS inhibited the expression of collagen I and α-SMA in LX-2 cells stimulated by TGF-β. Inhibition of lipid synthesis and promotion of oxidation by GPS were also confirmed in ethanol-treated AML12 cells. GPS could prevent hepatic steatosis advancing to the inception of a mild fibrosis caused by chronic alcohol exposure, suggesting GPS might be a promising therapy for targeting the early stage of alcoholic liver disease.
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To determine the relationship between the effect of wuzhi capsules on the blood concentration of tacrolimus as compared to diltiazem and with regard to cytochrome P450 (CYP)3A5 gene polymorphisms, 170 patients who underwent renal transplantation from November 2014 to March 2018 and used tacrolimus combined with diltiazem 30 mg bid were selected in this study retrospectively. Patients were divided into an observation group (105 patients) and a control group (65 patients) according to whether they used wuzhi capsules after the operation. The polymorphisms of CYP3A5*3 were determined and the effect of wuzhi capsules on the blood concentration of tacrolimus, as compared with that of diltiazem was determined in patients with different CYP3A5*3 genotypes. This study complies with relevant ethical norms. The results show that compared with diltiazem, an increase of tacrolimus C0/D was significantly correlated with the patient's CYP3A5*3 genotype in both the self-control and the control group. CYP3A5 expressers in the observation group were able to increase the tacrolimus C0/D by about 76.8% by replacing the wuzhi capsules with diltiazem, but this effect was not observed in CYP3A5 non-expressers. In CYP3A5 expressers wuzhi capsules had a greater ability relative to diltiazem to increase the blood concentration of tacrolimus.
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BACKGROUND@#Lung cancer is the cancer with the highest morbidity and mortality at home and abroad at present. Using computed tomography (CT) to screen lung cancer nodules is a huge workload. To test the effect of artificial intelligence in automatic identification of lung cancer by using artificial intelligence to find the lung cancer nodules automatically in the chest CT of 1 mm and 5 mm thick.@*METHODS@#5,000 cases of T1 stage lung cancer patients with 1 mm and 5 mm layer thickness were respectively labeled and learned by computer neural network, the algorithm of forming pulmonary nodules was carried out. 500 cases of chest CT in T1 stage lung cancer patients with 1 mm and 5 mm thickness were tested by artificial intelligence formation, and the sensitivity and specificity were compared with artificial reading.@*RESULTS@#Using artificial intelligence to read chest CT 500 in 5 mm, the sensitivity was 95.20%, the specificity was 93.20%, and the Kappa value of two times repeated read was 0.926,1. For 1 mm chest CT 500 cases, the sensitivity is 96.40%, the specificity is 95.60%, and the Kappa reads two times is 0.938,6. Compared with 5 doctors, the same CT sets with 1 mm thickness were read. The detection rates of artificial intelligence and artificial reading were similar to those of lung cancer nodules and negative control read films, and there was no significant difference between them. In the comparison of the same CT slices with 5 mm thickness, the number of detection of lung cancer nodules by artificial intelligence is better than that of artificial reading, and the sensitivity is higher, but the number of false messages is increased and the specificity is slightly worse.@*CONCLUSIONS@#The automatic learning of early lung cancer chest CT images by artificial intelligence can achieve high sensitivity and specificity of early lung cancer identification, and assist doctors in the diagnosis of lung cancer.
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Humans , Artificial Intelligence , Lung Neoplasms , Diagnosis , Pathology , Medical Informatics , Methods , Neoplasm StagingABSTRACT
Objective: To detect the expressions of periostin, matrix metalloproteinase-8 (MMP-8) and tissue inhibitor of matrix metalloproteinase-2 (TIMP-2) in gingival tissue of the patients with chronic periodontitis, and to explore the possible role of periostin in the occurrence and development of chronic periodontitis and its related biological mechanism. Methods: A total of 23 normal gingival tissues (control group) and 19 inflammatory gingival tissues (case group) were selected. The expressions of periostin, MMP-8 and TIMP-2 in gingival tissue of the patients in two groups were detected by immunohistochemical SP method. The correlation between the expressions of periositn and MMP-8 and TIMP-2 in gingival tissue of the patients in two groups were detected by Spearman analysis. Results: The positive expression rate of periostin in gingival tissue of the patients in case group was lower than that in control group (P<0. 05). The correlative analysis results demonstrated that the expression of periostin had negative relationship with the expression of MMP-8 (r=-0.51, P<0. 05) in control group, and positive relationship with the expression of TIMP-2 (r=0. 50, P<0. 05); in case group, the expression of periostin had negative relationship with the expression of MMP-8 (r=-0.482, P<0. 05), and positive relationship with the expression of TIMP-2 (r= 0.655, P<0. 05). Conclusion: Periostin can be used as a preliminary indicator for maintaining the structural stability of gingival tissue, and become a research target for periodontal tissue regeneration.
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Objective To evaluate laparoscopic surgery for Mirizzi syndrome.Methods A retrospective study was carried out on 14 patients undergoing laparoscopic operation for Mirizzi syndrome from Feb.2013 to Nov.2018.There were 4 patients of type Ⅰ,8 patients of type Ⅱ,2 patients of type Ⅲ according to Csendes classification.Results Laparoscopic procedures were successfully completed in all patients.All 4 cases of type Ⅰ received laparoscopic cholecystectomy.4 patients of type Ⅱ were given laparoscopic cholecystectomy combined with primary suture of common bile duct;the other 4 patients of type Ⅱ underwent laparoscopic cholecystectomy plus T tube drainage.2 patients of type Ⅲ were treated with laparoscopic partial-cholecystectomy combined with repair of the duct defect and T tube drainage.All the patients recovered uneventfully.The postoperative hospitalization time was 4 to 7 d,with an average of 5.5 d.There were no major postoperative complications.14 patients were followed up for 1-8 months,and there was no biliary stricture and no stone recurrences.Conclusion For cases of type Ⅰ,type Ⅱ and type Ⅲ,laparoscopic surgery is a safe treatment.
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The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.
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Antioxidants , Chemistry , Pharmacology , Flowers , Chemistry , Hibiscus , Chemistry , Monosaccharides , Chemistry , Phytochemicals , Chemistry , Pharmacology , Pigmentation , Polysaccharides , Chemistry , PharmacologyABSTRACT
Objective:To investigate the treatment and aesthetic restoration of anterior tooth loosening and falling in the patient with severe periodontitis,and to clarify the curative effect of multidisciplinary treatment in the anterior esthetic zone,and to provide reference for its clinical application.Methods:The male patients was 57 years old,and saw a dcotor becasuse of anterior teeth loosening and discomfort for more than half a year.The specialized examination:11,21,22,41 Ⅲ°loose,42 Ⅱ°loose.After multidisciplinary consultation,the teeth were treated with periodontal therapy.After inflammation was controlled,11,21,22,41,42 were removed for immediate implantation.The platelet rich fibrin(PRF)combined with guided bone regeneration(GBR)was used to repair the bone defect around the implants.Because of the aesthetic requirements of the patients,tooth preparation was performed for 12,13,23,31,32,33,43 after root canal therapy.Fixed restorations were performed in 13-23 and 33-43.Results:After operation,the implant was well positioned and the bone surrounding the implant was adequate.The soft tissue in the mouth healed well and the degree of the alveolar ridge was better.After restoration, the masticatory and phonation function of the teeth were restored,and the patients were satisfied with the aesthetic effect.Conclusion:Through the application of multidisciplinary treatment,the implant repair of the anterior esthetic zone of the patient with severe periodontitis is successfully achieved,which opens a new way for the diagnosis and treatment of the anterior esthetic zone.
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Objective:To discuss the therapeutic effect of related periodontal surgical treatment and aesthetic rerepair in the patient with residual root and crown of anterior teeth induced by unfitting prosthesis and to clarify the curative effect of periodontal surgical treatment and aesthetic re-repair in the anterior teeth area,and to provide guidance for making the program of clinical treatment.Methods:Two years after repair of the anterior teeth of a patient,the restorations loosed;on the one hand the patient had a toothache of chewing food accompanying gingival bleeding,on the other hand the upper labial frenum was too high,furthermore the upper left incisor apical area showed low density images.After the consultation of periodontics,endodontics,prosthodontics and other disciplines doctors,anterior teeth root canal re-treatment and periodontal basic treatment were adopt as well ascrown lengthening combined with labial freneetomy to restore the normal biological width,additionally apply not only guided bone regeneration (GBR) but also platelet rich fibrin (PRF) to repair the defects of apical bone.One month later,provisional restorations were put into use and then three months later permanent repairs were conducted.Results:The corresponding gingival and gingival papilla shape recoverd perfectly and the height of gingiva coordinated with that of adjacent teeth,in the meantime the upper labial frenum attached normally and there was not obvious space between upper incisors;the morphology and function examination of restoration in maxillary anterior region were both well.The follow-up X-ray showed that the bone density of the defect area was the same as that of the peripheral bone without obvious boundaries.Conclusion:The aesthetic repair of thin biotype referring to anterior area,especially in the complicated re-treatment cases,the intervention of the appropriate periodontal surgical treatment is not only beneficial to obtain the obvious short-term effect,but also positive to stabilize longterm effects.
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Objective:To explore the influence of gallnut extract as a root canal irrigants in the curative effect of chronic periapical periodontitis,and to clarify its mechanism.Methods:A total of 192 patients diagnosed with chronic periapical periodontitis were selected and randomly divided into control group (distilled water),hydrogen peroxide group,sodium hypochlorite group and gallnut extract group (n=48).In the process of root canal treatment,four irrigants were used respectively,then the curative effects 1 week,6 months,12 months after treatment were observed and compared.Results:After root filling treatment,the cold and heat stimulation of the tooth was insensitive in clinical examination,percussion (-),loose degree≤ Ⅰ °,no swollen gums,bleeding on probing (-),no fistula,normal function of the exercise of chewing,decreased or disappeared transmission area in periapical bone in X-ray photo,and those metioned above were considered effective treatment.After 6 months oftreatment,the effective rate in gallnut group was 89.4％,and there were no statistically significant differences compared with hydrogen peroxide group (91.1％) and sodium hypochlorite group (91.5 ％) (P＞0.05),but there was significant difference compared with control group (67.4％) (P＜0.05).After root filling treatment for 12 months,the effective rate in gallnut group was 83.7％ and there were no statistically significant differences compared with hydrogen peroxide group (83.3％)and sodium hypochlorite group (87.8％) (P＞0.05),but the ratio of tooth from improvement to recovery in gallnut group was higher than those in hydrogen peroxide group and sodium hypochlorite group.Conclusion:Gallnut extract as a root canal irrigants has good antibacterial and cleaning effect,and can achieve the aim of curing the chronic root periapical inflammation and preventing the infection.
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Objective; To analyze the clinical manifestion and treatment method of one patient with severe periodontitis complicated with oral lichen planus, and to clarify the effect of combined treatment method and the importance of regular follow-up. Methods; The clinical manifestion of the patient was that the bilateral moral teeth had become looseness and the gum had bled for six months, and the patients had a history of oral lichen planus in gum for 2 years old. The probing depth (PD) of all the teeth was 5-6 mm and the attachment loss (AL) was 8-9 mm, with II °-IE "mobility and bleeding on probing (BOP) (+). The teeth was treated with the regular basis periodontal treatment combined with gingival triamcinolone acetonide injection treatment. When the inflammation situation remained stable, the delayed implantation was performed and the crown restoration treatment was done for 26, 27, 36 and 37. The splinting treatment was done for 31 and 41 II mobile teeth. The regular follow-up periods were 1 week, 2 weeks, 1 month, 3 months, and 6 months after treatment during periodontal maintenance. Results; After combined treatment and regular follow-up, the PD of both anterior and premolar were 4 mm, and the BOP was negative. The clinical symptom of oral lichen planus of the patient was relieved, and there were no symptoms of congestion and erosion on the gum. After the inflammation situation was controlled, the chewing function of the left molar region was restored by implantation rehabilitation. A 3-month-follow-up cycle and excellent treatment compliance were beneficial for the control of disease. The optimal follow-up period of gingival condition maintained best was once per 2 weeks. Conclusion: The combined treatment can relieve the clinical symptoms of the patient with severe periodontitis complicated with oral lichen planus. Regular follow-up and excellent treatment compliance are conducive to controlling the condition of disease.
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Objective:To investigate the effect of Chinese traditional medicine-Shuanghuangbu combined with Xipayi Mouth Rinse on the periodontal indexes of the peri-implantitis patients,and to detect the curative effect of Shuanghuangbu combined with Xipayi Mouth Rinse in the treatment of peri-implantitis.Methods: A total of 40 patients diagnosed as peri-implantitis were randomly divided into control group and Chinese traditional medicine group (n=20).All of the patients received supragingival scaling and subgingival scaling,and then the peri-implant pocket of the patients in Chinese traditional medicine group were washed with Xipayi Mouth Rinse,while the fine probe slided slowly along the inner wall of peri-implant pocket into the bottom and retained the Shuanghuangbu,and retreated gently and made the medicine overflow the gingival margin.The patients in control group were given 3% hydrogen peroxide and 0.9% sodium chloride injection washed by turns.The indexes of covering modified plaque index (mPLI),probing pocket depth (PPD),modified sulcular bleeding index (mSBI)of the patients in two groups at baseline and after treatment were detected;Whatman# 1 was applied to aspirate the peri-implant sulcus fluid (PISF)which were weighed at baseline and after treatment,and the level of interleukin-1β (IL-1β)in PISF was analyzed by ELISA method.Results:No differences were observed in age,sex,periodontal indexes, weights of PISF and IL-1βlevels of the patients in two groups before treatment (P >0.05).Compared with before treatment,the indexes including PPD,mSBI,mPLI,weight of PISF and IL-1βlevel in PISF of the patients in two groups were decreased after treatment (P < 0.05).After treatment,the differences in mSBI,mPLI,weight of PISF and IL-1β level in PISF of the patients between control group and Chinese traditional medicine group were significant (P <0.05).Compared with control group,the PPD level of the patients in Chinese traditional medicine group was decreased,but the difference was not significant (P >0.05).Conclusion:The curative effect of Chinese traditional medicine-Shuanghuangbu combined with Xipayi Mouth Rinse is obsious,and the treatment can improve the periodontal status and reduce the level of IL-1βin PISF.
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Objective To investigate the protective effect of curcumin pretreatment on the intestinal mucosa in rats in dry and hot desert environment. Methods Eighty male SPF grade rats were randomly divided into saline control group ( NC group) and curcumin pretreatment group ( HDC group ) ( 40 in each group ) . Rats in the NC group were gavaged with saline, and rats in the HDC group were gavaged with curcumin (200 mg/kg), once a day for 7days. The rats were placed in a cabin simulating the dry and hot desert environment (41 ± 0. 5)℃ and relative humidity of (10 ± 2)%. Rats were randomly taken from each groups ( n=10 ) and sacrificed with 3% sodium pentobarbital intraperitoneally at 0 min, 50 min, 100 min and 150 min time points. The ileal tissue was stained for histological examination and oxidative stress index was detected. Results At time points 0 min and 50 min, the pathological injury scores of the HDC group were not significantly different compared with the NC group (P > 0. 05). At time points 100 min and 150 min, the pathological injury scores of the HDC group were significantly decreased compared with the NC group ( P < 0. 01 ) . At time points 50 min, 100 min and 150 min, the CAT and SOD activity of HDC group were significantly increased compared with the NC group (P< 0. 05 or P< 0. 01). The MDA content of HDC group were significantly decreased compared with the NC group ( P< 0. 05 or P < 0. 01 ) . Exposed to dry and hot environment, the pathological injury scores of the NC group were negatively correlated with CAT and SOD activity (r= -0. 9128, r= -0. 9125, P< 0. 01), and positively correlated with MDA content (r=0. 9258, P< 0. 01). Conclusions Curcumin pretreatment can protect the intestinal mucosa of rats in dry-heat environment of desert, and curcumin can alleviate the pathological damages of intestinal mucosa by inhibiting the oxidative stress in intestinal mucosa.
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Objective To study the role of arrestin domain-containing protein 4( ARRDC4) in regulation of enterovirus 71(EV71) triggered innate IL-6 production and the underlying mechanism .Methods THP-1-derived macrophages (t-M?) were transfected with ARRDC4 specific siRNA and negative control siRNA .The expression and production of IL-6, replication and virus titer of EV71, and the activation of signaling pathway adaptors were analyzed with quantitative real -time PCR, ELISA and Western blot.Results Upon EV71 infection, ARRDC4 was upregulated.ARRDC4 silencing could enhance mRNA expression and production of IL-6, thus increasing the replication and virus titer of EV71.In ARRDC4 silenced t-M?, the activation of p-65,IκBα,ERK,JNK and p38 was promoted.Conclusion ARRDC4 promotes EV71-t riggered IL-6 production by enhancing the activation of NF-κB and MAPK signaling pathway to inhibit EV71 infection, contributing to positive regulation of anti-EV71 innate immune responses .
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Objective To study the role of arrestin domain-containing protein 4( ARRDC4) in regulation of enterovirus 71(EV71) triggered innate IL-6 production and the underlying mechanism .Methods THP-1-derived macrophages (t-M?) were transfected with ARRDC4 specific siRNA and negative control siRNA .The expression and production of IL-6, replication and virus titer of EV71, and the activation of signaling pathway adaptors were analyzed with quantitative real -time PCR, ELISA and Western blot.Results Upon EV71 infection, ARRDC4 was upregulated.ARRDC4 silencing could enhance mRNA expression and production of IL-6, thus increasing the replication and virus titer of EV71.In ARRDC4 silenced t-M?, the activation of p-65,IκBα,ERK,JNK and p38 was promoted.Conclusion ARRDC4 promotes EV71-t riggered IL-6 production by enhancing the activation of NF-κB and MAPK signaling pathway to inhibit EV71 infection, contributing to positive regulation of anti-EV71 innate immune responses .
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Objective:To observe the repair effect of nano hydroxyapatite/magnesium(nHA-Mg)porous composite material modified by poly lactic acid/glycolic acid copolymer(PLGA) on the jaw bone defect of rabbits,and to elucidate the mechanisms preliminary.Methods:The mandibular defect models of 10 mm×5 mm×1 mm in the 18 rabbits were established.Nine rabbits were selected and implanted with nHA-Mg composite materials modified by PLGA in the left as experimental group and the right as blank control group;the other nine were implanted with nHA-Mg in the left as positive control group and the right as blank control group.The rabbits were sacrificed at 4, 8, 12 weeks (respectively 3 of experimental group and positive control group every time)and the mandibular defect areas were intercepted and observed by imaging and histological examination;the sizes of the newborn trabecula area and residual materials in experimental group and positive control group were compared.Results:Compared with positive control group and blank control group, the percentage of newborn trabecula area of the rabbits in experimental group was increased (P0.05).The imaging results showed that the new bone formation can be observed in experimental group and better than positive control group and blank control group.The paraffin section results indicated that there were visible vascular tissue and newborn trabecula,the osteoblasts gathered around the bone trabecula;a lot of pits were located in the bone trabecula, and the pits contained osteocytes in experimental group.As the prolongation of time,thickened newborn trabecula, dense arrangement and trend of converting to lamellar bone were observed in experimental group.The hard tissue section results showed that the remaining amount of materials in experimental group was more than that in positive control group.Conclusion:nHA-Mg porous composite materials modified by PLGA can effectively reduce the rate of degradation in the body, promote osteogenesis and guide the bone regeneration.
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<p><b>OBJECTIVE</b>To investigate the changes in the range of motion (ROM) and stress of the intervertebral disc and facet joint of the adjacent segments following anterior cervical corpectomy and fusion (ACCF) and anterior cervical discectomy and fusion (ACDF) using finite element analysis.</p><p><b>METHODS</b>A three-dimensional finite element model of the lower cervical vertebrae was constructed and validated by comparing the ROM of the finite element model against the published data. After the validation of successful modeling, finite element models of ACDF and ACCF were constructed. The ROM and the stress of the intervertebral disc and facet joint of the adjacent segments were compared between the intact lower cervical vertebrae and the cervical vertebrae after ACDF and ACCF.</p><p><b>RESULTS</b>The ROM of the finite element model was consistent with the published data. The total ROM and the ROM of the fusion segments with ACDF and ACCF were significantly decreased compared with the intact cervical vertebrae. In the adjacent segments following ACDF and ACCF, the ROM the adjacent segments and the stress peak of the intervertebral disc and facet joint all increased obviously compared with those of intact cervical vertebrae.</p><p><b>CONCLUSION</b>After fusion surgeries, the total ROM of the cervical vertebrae decreases and the ROM of the adjacent segment increases. The stress peak of the intervertebral disc and facet joint of the adjacent segments also increases to significantly alter the physiological characteristics of the intact cervical vertebrae.</p>
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According to the technology requirements of the fourth national survey of Chinese Materia Medica resources (pilot), suitable investigation method of exploration and suggestions for investigating Chinese Materia Medica resources was proposed based on the type of wetland and artificial water of Hongze Lake region. Environment of Hongze Lake and overview of wetland, present situation of ecology and vegetation and vegetation distribution were analyzed. Establishment of survey plan, selection of sample area and sample square and confirmation of representative water area survey plan were all suggested. The present study provide references for improving Chinese materia medica resources survey around Hongze Lake, and improving the technical specifications. It also provide references for investigating Chinese Materia Medica resources survey on similar ecological environment under the condition of artificial intervention.
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<p><b>OBJECTIVE</b>To retrospectively analyze the surgical methods and its clinical effects and explore a clinical classification and treatment strategy for atlantoaxial dislocation(AAD).</p><p><b>METHODS</b>The clinical data of 89 patients with atlantoaxial dislocation were analyzed from September 2005 to September 2013. There were 49 males and 40 females, aged from 13 to 67 years with an average of 48.1 years. According to the reductive effects with preoperative cervical dynamic radiograph and high weight skeletal traction under general anesthesia, the dislocations were classified into three types:easy reduction type, hard reduction type and irreducible type. The patients with easy reduction type were treated with posterior screw rod internal fixation after manual reduction, while the patients with hard reduction type were treated with posterior screw rod fixation after high weight skeletal traction reduction under general anesthesia. The patients with irreducible type were treated with transoral atlantoaxial joint release or depression and posterior internal fixation and fusion. According to JOA scores to evaluate the neurological status and treatment outcome.</p><p><b>RESULTS</b>Thirty patients were classified as easy reduction type, 55 patients as hard reduction type, and 4 patients as irreducible type. The preoperative JOA score was 8.2±3.1 on average, while the postoperative score was 14.2±2.4. The improvement rate was 40.1% to 82.5% with an average of 62.5%. Eighty nine patients were followed up from 6 to 37 months with a mean of 17.3 months. Eighty two cases obtained anatomical reduction and 85 cases obtained bony fusion. One case complicated with hyponatremia after operation and 1 case combined with Guillain-Barre syndrome, 4 cases complicated with delayed union wounds, 1 case died of for respiratory failure 2 years after operation. No wound infections were found in the patients approach for transoral operation.</p><p><b>CONCLUSIONS</b>According to the cervical dynamic radiograph and high weight skeletal traction under general anesthesia to classify for atlantoaxial dislocation, and adopting well strategies to treat the patients, can achieve satisfactory effects.</p>
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·AlM:To study the MRG15 (death factor related gene) in age-related cataract ( ARC ) , differential expression of normal lens epithelial cells. · METHODS: Forty mature healthy female SD rats were randomly divided into study group and control group, 20 rats in each group, the study group were ovariectomized, low concentration of naphthalene long interval of administration, the establishment of perimenopausal ARC model, the control group of conventional farming.ln the subtractive hybridization cloning by MRG15, and make the probe of the cDNA fragment using digoxigenin labeled. Access to the two groups of rats anterior lens capsule after slicing, and then through the differential expression in situ hybridization clear lens epithelial cells. · RESULTS: The cloned MRG15 through BamH1, EcoR1 enzyme digestion and agarose gel electrophoresis, available for 639bp long cDNA fragment. GeneBank display contrast, their homology was 99.0%. ln situ hybridization, ARC patients and normal lens epithelial cells were observed in the expression of MRG15.Study group the percentage of positive cells compared with control group, showed a significant difference (P<0.05). lntegral index study group and the control group compared with, was significantly difference (P<0.05). · CONCLUSlON: ARC, MRG15 in normal lens epithelial cells expressed ARC, and compared with the normal expression of lens epithelial cells, which may produce inhibitory effects are associated with MRG15 transcription in human lens epithelial cells in the part of key genes, by reducing the lens epithelial cell fu nction, make its appear aging, and the formation of cataract, clinical response to this should further study.